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Introduction Pipeline Advantages Sample Typical Cases

The eukaryotic transcriptome sequencing is based on the HiSeq platform to sequence all the mRNAs transcribed by eukaryotic specific tissues or cells at a certain time. Both genes can be studied and new genes can be explored to obtain complete and rapid mRNA sequences and abundance information. According to the presence or absence of reference genome, eukaryotic transcriptase sequencing can be divided into: reference transcriptome, no reference transcriptome.

Sequencing strategy:

(1)Eukaryotic reference transcriptome sequencing 

Sequencing platform:Hiseq4000
Sequencing type:PE150
Library type:200bp
Data:4~10Gb clean data

(2)Eukaryotic non-reference transcriptome sequencing 

Sequencing platform:Hiseq4000
Sequencing type:PE150
Library type:200bp
Data:8~12Gb clean data

(3)Digital gene expression profiles upgraded

Sequencing platform:Hiseq2500
Sequencing type:SE50
Library type:200bp
Data:6~20Gb clean reads